Composite

Part:BBa_K1460003:Design

Designed by: Eric Holmes   Group: iGEM14_Cornell   (2014-08-14)


Anderson Promoter + NixA + Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Anderson promoter provides strong constitutive expression of NixA in E. coli.


Source

Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. NixA gene is naturally found in H. pylori.[1] This part was synthesized by GenScript in the vector pUC57. Part was then transferred into pSB1C3 using standard cloning procedures.

References

[1] Mobley, H., Garner, R., & Bauerfeind, P. (1995). Helicobacter pylori nickel-transport gene nixA: Synthesis of catalytically active urease in Escherichia coli independent of growth conditions. Molecular Microbiology, 97-109.